After sonification, the precipitate was washed repeatedly by ionic and non-ionic detergents to remove membrane proteins as could as possible, and the cleaner inclusion bodies were abtained 其次對包涵體體外復性進行研究。用離子型與非離子型去污劑反復洗滌包涵體,盡可能去除膜蛋白。
One small captive population of amur tigers ( panthera tigris altica ), which had 14-year captive history since its foundation in 1986, was selected for such population genetic researches in this paper, which contained three experimental parts as : ( 1 ) dna extraction from shed hairs . three methods, which were chelex, ionic detergent and non-ionic detergent, were respectively used to digest the shed hairs of one amur tiger 本文利用chelex、離子型和無離子型分解液三種方法對在東北虎(pantheratigrtsaltica)換毛期采集的自然脫落毛發(fā)進行了dna提取,并對部分毛發(fā)分解原液進行蛋白質沉淀,對于毛發(fā)分解殘留物也進行了核細胞分解與蛋白質沉淀再處理,對上述提取產(chǎn)物進行rapd擴增。
One small captive population of amur tigers ( panthera tigris altica ), which had 14-year captive history since its foundation in 1986, was selected for such population genetic researches in this paper, which contained three experimental parts as : ( 1 ) dna extraction from shed hairs . three methods, which were chelex, ionic detergent and non-ionic detergent, were respectively used to digest the shed hairs of one amur tiger 本文利用chelex、離子型和無離子型分解液三種方法對在東北虎(pantheratigrtsaltica)換毛期采集的自然脫落毛發(fā)進行了dna提取,并對部分毛發(fā)分解原液進行蛋白質沉淀,對于毛發(fā)分解殘留物也進行了核細胞分解與蛋白質沉淀再處理,對上述提取產(chǎn)物進行rapd擴增。